伟德体育在线

组伟德体育在线修饰抗体

表观遗传学是研究基因表达中可遗传的表型变化,包括修饰DNA,RNA和伟德体育在线质,但不涉及核苷酸序列的改变。翻译后修饰(PTM)是研究伟德体育在线质表观遗传学的最重要方法。PTM是标记,为细胞提供广泛的调节机制,以指示哪些基因可以关闭和打开。许多类型和家族的伟德体育在线质都受到PTM的影响,但得到最多修饰的伟德体育在线质之一是组伟德体育在线家族。

组伟德体育在线将基因组DNA包装到核小体中,这使得可将大约2米的DNA放入细胞的细胞核。核小体含有两个亚基,每个亚基由组伟德体育在线H2A,H2B,H3和H4组成。此外,还有组伟德体育在线H1,通常被称为连接组伟德体育在线。在组伟德体育在线上发现的最普遍的PTM包括甲基化,乙酰化,磷酸化和泛素化,这些修饰在基因表达中至关重要,可用作打开和压缩染色质的信号,以及促进和拮抗转录的募集因子。

The Schematic of Common Histone Modification Site

这些组伟德体育在线修饰可通过调节基因表达影响染色体功能。华美生物可提供高特异性组伟德体育在线修饰抗体。可用于ChIP、WB、IHC、IP等多种实验类型。

伟德体育在线优势

  • 高特异性

Western Blot analysis of Acetyl-HIST1H1C (K84) antibody (CSB-PA010378OA84acHU)

Western Blot analysis of 2-hydroxyisobutyryl-HIST1H1C (K109) Antibody (CSB-PA010378OA109hibHU)

Western Blot analysis of β-hydroxybutyryl-HIST1H3A (K18) Antibody (CSB-PA010418OA18bhbHU)

  • 可用于ChIP实验

Chromatin Immunoprecipitation (ChIP) analysis of Acetyl-HIST1H1C (K84) antibody (CSB-PA010378OA84acHU)

Chromatin Immunoprecipitation (ChIP) analysis of 2-hydroxyisobutyryl-HIST1H1C (K109) Antibody (CSB-PA010378OA109hibHU)

Chromatin Immunoprecipitation (ChIP) analysis of β-hydroxybutyryl-HIST1H3A (K18) Antibody伟德体育在线 (CSB-PA010418OA18bhbHU)

伟德体育在线列表

伟德体育在线部分热销组伟德体育在线修饰抗体列表(见附表一)

靶点 伟德体育在线名称 靶点 伟德体育在线名称
H1F0 (K101) Mono-methyl-H1F0 (K101) Antibody HIST1H3A (K18) 2-hydroxyisobutyryl-HIST1H3A (K18) Antibody
H3F3A (K79) Di-methyl-H3F3A (K79) Antibody Butyrly-HIST1H3A (K18) Antibody
HIST1H1C (Ab-109) HIST1H1C (Ab-109) Antibody β-hydroxybutyryl-HIST1H3A (K18) Antibody
HIST1H1C (Ab-167) HIST1H1C (Ab-167) Antibody 2-hydroxyisobutyryl-HIST1H3A (K18) Antibody
HIST1H1C (Ab-186) HIST1H1C (Ab-186) Antibody HIST1H3A (K23) β-hydroxybutyryl-HIST1H3A (K23) Antibody
HIST1H1C (Ab-45) HIST1H1C (Ab-45) Antibody Butyrly-HIST1H3A (K23) Antibody
HIST1H1C (Ab-96) HIST1H1C (Ab-96) Antibody Propionyl-HIST1H3A (K23) Antibody
HIST1H1C (K109) Formyl-HIST1H1C (K109) Antibody HIST1H3A (K27) 2-hydroxyisobutyryl-HIST1H3A (K27) Antibody
2-hydroxyisobutyryl-HIST1H1C (K109) Antibody Acetyl-HIST1H3A (K27) Antibody
HIST1H1C (K158) 2-hydroxyisobutyryl-HIST1H1C (K158) Antibody Butyrly-HIST1H3A (K27) Antibody
HIST1H1C (K186) Mono-methyl-HIST1H1C (K186) Antibody β-hydroxybutyryl-HIST1H3A (K27) Antibody
HIST1H1C (K26) 2-hydroxyisobutyryl-HIST1H1C (K26) Antibody Tri-methyl-HIST1H3A (K27) Antibody
HIST1H1C (K45) Mono-methyl-HIST1H1C (K45) Antibody HIST1H3A (K36) Acetyl-HIST1H3A (K36) Antibody
Di-methyl-HIST1H1C (K45) Antibody Mono-methyl-HIST1H3A (K36) Antibody
HIST1H1C (K84) Acetyl-HIST1H1C (K84) Antibody Mono-methyl-HIST1H3A (K36) Antibody
HIST1H1C (K96) Acetyl-HIST1H1C (K96) Antibody Tri-methyl-HIST1H3A (K36) Antibody
Formyl-HIST1H1C (K96) Antibody HIST1H3A (K37) Acetyl-HIST1H3A (K37) Antibody
Mono-methyl-HIST1H1C (K96) Antibody HIST1H3A (K4) 2-hydroxyisobutyryl-HIST1H3A (K4) Antibody
HIST1H1D (Ab-140) HIST1H1D (Ab-140) Antibody β-hydroxybutyryl-HIST1H3A (K4) Antibody
HIST1H1E (Ab-25) HIST1H1E (Ab-25) Antibody Tri-methyl-HIST1H3A (K4) Antibody
HIST1H1E (T17) Phospho-HIST1H1E (T17) Antibody Tri-methyl-HIST1H3A (K4) Antibody
HIST1H2AG (K118) Formyl-HIST1H2AG (K118) Antibody HIST1H3A (K56) Acetyl-HIST1H3A (K56) Antibody
HIST1H2AG (K74) 2-hydroxyisobutyryl-HIST1H2AG (K74) Antibody Malonyl-HIST1H3A (K56) Antibody
Acetyl-HIST1H2AG (K74) Antibody HIST1H3A (K79) β-hydroxybutyryl-HIST1H3A (K79) Antibody
HIST1H2AG (K9) Acetyl-HIST1H2AG (K9) Antibody Butyrly-HIST1H3A (K79) Antibody
HIST1H2BC (K108) 2-hydroxyisobutyryl-HIST1H2BC (K108) Antibody HIST1H3A (K9) Acetyl-HIST1H3A (K9) Antibody
HIST1H2BC (K11) Acetyl-HIST1H2BC (K11) Antibody Crotonyl-HIST1H3A (K9) Antibody
β-hydroxybutyryl-HIST1H2BC (K11) Antibody Butyrly-HIST1H3A (K9) Antibody
HIST1H2BC (K116) Formyl-HIST1H2BC (K116) Antibody Di-methyl-HIST1H3A (K9) Antibody
Acetyl-HIST1H2BC (K116) Antibody HIST1H3A (T3) Phospho-HIST1H3A (T3) Antibody
Mono-methyl-HIST1H2BC (K116) Antibody HIST1H4A (K12) Succinyl-HIST1H4A (K12) Antibody
HIST1H2BC (K12) Acetyl-HIST1H2BC (K12) Antibody Formyl-HIST1H4A (K12) Antibody
2-hydroxyisobutyryl-HIST1H2BC (K12) Antibody Butyrly-HIST1H4A (K12) Antibody
Mono-methyl-HIST1H2BC (K12) Antibody 2-hydroxyisobutyryl-HIST1H4A (K12) Antibody
HIST1H2BC (K120) 2-hydroxyisobutyryl-HIST1H2BC (K120) Antibody HIST1H4A (K16) Butyrly-HIST1H4A (K16) Antibody
Glutaryl-HIST1H2BC (K120) Antibody Propionyl-HIST1H4A (K16) Antibody
HIST1H2BC (K15) Acetyl-HIST1H2BC (K15) Antibody 2-hydroxyisobutyryl-HIST1H4A (K16) Antibody
Mono-methyl-HIST1H2BC (K15) Antibody HIST1H4A (K20) Acetyl-HIST1H4A (K20) Antibody
HIST1H2BC (K16) Crotonyl-HIST1H2BC (K16) Antibody HIST1H4A (K31) Propionyl-HIST1H4A (K31) Antibody
HIST1H2BC (K20) Crotonyl-HIST1H2BC (K20) Antibody 2-hydroxyisobutyryl-HIST1H4A (K31) Antibody
Butyrly-HIST1H2BC (K20) Antibody Acetyl-HIST1H4A (K31) Antibody
2-hydroxyisobutyryl-HIST1H2BC (K20) Antibody HIST1H4A (K5) Butyrly-HIST1H4A (K5) Antibody
Acetyl-HIST1H2BC (K20) Antibody Propionyl-HIST1H4A (K5) Antibody
β-hydroxybutyryl-HIST1H2BC (K20) Antibody 2-hydroxyisobutyryl-HIST1H4A (K5) Antibody
Mono-methyl-HIST1H2BC (K20) Antibody β hydroxybutyryl-HIST1H4A (K5) Antibody
HIST1H2BC (K24) Acetyl-HIST1H2BC (K24) Antibody HIST1H4A (K59) Mono-methyl-HIST1H4A (K59) Antibody
HIST1H2BC (K34) 2-hydroxyisobutyryl-HIST1H2BC (K34) Antibody HIST1H4A (K77) Formyl-HIST1H4A (K77) Antibody
HIST1H2BC (K5) Butyrly-HIST1H2BC (K5) Antibody 2-hydroxyisobutyryl-HIST1H4A (K77) Antibody
HIST1H2BC (S14) Phospho-HIST1H2BC (S14) Antibody Acetyl-HIST1H4A (K77) Antibody
HIST1H3A (K115) Acetyl-HIST1H3A (K115) Antibody HIST1H4A (K79) Formyl-HIST1H4A (K79) Antibody
HIST1H3A (K122) 2-hydroxyisobutyryl-HIST1H3A (K122) Antibody HIST1H4A (K8) Butyrly-HIST1H4A (K8) Antibody
Formyl-HIST1H3A (K122) Antibody Propionyl-HIST1H4A (K8) Antibody
Acetyl-HIST1H3A (K122) Antibody 2-hydroxyisobutyryl-HIST1H4A (K8) Antibody
HIST1H4A (K91) Acetyl-HIST1H4A (K91) Antibody
β-hydroxybutyryl-HIST1H4A (K91) Antibody

关于组伟德体育在线修饰

组伟德体育在线甲基化是通过添加甲基来修饰组伟德体育在线中的某些氨基酸,继而抑制或激活转录。组伟德体育在线甲基转移酶(HMT)通过染色质依赖性转录抑制或激活来控制或调节DNA甲基化。组伟德体育在线的甲基化和去甲基化在DNA中打开和关闭基因。

组伟德体育在线磷酸化伟德体育在线是指激酶使组伟德体育在线上的特异性丝氨酸、苏氨酸或酪氨酸磷酸化,并且可通过磷酸酶进行去磷酸化。磷酸化通常发生在DNA修复和有丝分裂期间,另外在细胞分裂和细胞凋亡过程的DNA修复、转录和染色质整合中起重要的作用。

组伟德体育在线乙酰化伟德体育在线主要发生在H3、H4的N端相对保守的赖氨酸位置上,是由组伟德体育在线乙酰转移酶和组伟德体育在线去乙酰化酶协调进行。该过程与许多细胞过程的调控密切相关,包括染色质动力学和转录,基因沉默,细胞周期进程,细胞凋亡、分化、DNA复制、DNA修复、核输入和神经元抑制。

组伟德体育在线琥珀酰化是一种翻译后修饰,将琥珀酰基(-CO-CH2 -CH2 -COOH)加到组伟德体育在线的赖氨酸残基上。琥珀酰化的潜在作用正在研究中,但随着琥珀酰基的加入,赖氨酸的电荷从+1变为-1(在生理pH下)并引入一个100Da结构,明显大于乙酰化(42Da)或甲基化(14 Da),研究人员推测这将会使伟德体育在线质结构和功能发生更显着的变化。

组伟德体育在线2-羟基异丁酰化是一种新型的组伟德体育在线标记,并已在63个人和小鼠组伟德体育在线的Khib位点得到鉴定,包括27个独特的赖氨酸位点。该组伟德体育在线标记最初由MS鉴定,然后通过化学和生物化学方法验证。

组伟德体育在线生物素化伟德体育在线是一个相对较新的研究领域,组伟德体育在线生物素化的生物学作用证据很少。最新研究表明,组伟德体育在线生物素化可以增强人淋巴细胞中细胞增殖的反应。此外,组伟德体育在线生物素化也可能在细胞对DNA损伤的反应中起重要作用。

组伟德体育在线甲酰化伟德体育在线也是翻译后修饰之一,可以调节染色质构象和基因激活。目前已经在组伟德体育在线中赖氨酸残基的Nε上得到鉴定。另外也在接头组伟德体育在线和高迁移率族伟德体育在线中观察到这种修饰。

组伟德体育在线巴豆酰化伟德体育在线是一种新确定的组伟德体育在线修饰,与哺乳动物细胞中的活跃转录有关。赵英明教授实验室于2011年发现一种新的赖氨酸残基组伟德体育在线修饰物,该修饰与基因表达相关,并且标记了启动子和增强子,类似于乙酰化,后命名为巴豆酰化。

组伟德体育在线羟基化伟德体育在线是将CH基团转化为COH基团的许多翻译后修饰之一。羟基化是一种氧化过程。一些研究表明,组伟德体育在线5-羟基化可能在基因转录或染色体重排的表观遗传调控中起重要作用。

组伟德体育在线丙二酰化是一种新型的组伟德体育在线PTM。相关研究表明,与赖氨酸乙酰化相比,赖氨酸丙二酰化将带正电的残基变为负电荷,这将会引起更显着的结构变化。

组伟德体育在线戊二酸化伟德体育在线通常发生在赖氨酸上。Kristin A. Anderson等人已经鉴定并全面验证赖氨酸戊二酸化在进化过程中属于保守的PTM,主要影响代谢过程和其他线粒体功能。

组伟德体育在线β-羟基丁基化是一种新型的组伟德体育在线修饰,通常也发生在赖氨酸上。谢中宇团队的研究表明,小鼠肝脏长时间禁食会显着诱导β-羟基丁基化,与饥饿反应代谢途径中表达上调的基因相关。